Article
High-throughput resequencing of target-captured cDNA in cancer cells.
Cancer science - 1 Jan 2012
Ueno Toshihide, Yamashita Yoshihiro, Soda Manabu, Fukumura Kazutaka, Ando Mizuo, Yamato Azusa, Kawazu Masahito, Choi Young Lim, Mano Hiroyuki
Abstract excerpt
The recent advent of whole exon (exome)-capture technology, coupled with second-generation sequencers, has made it possible to readily detect genomic alterations that affect encoded proteins in cancer cells. Such target resequencing of the cancer genome, however, fails to detect most clinically-relevant gene fusions, given that such oncogenic fusion genes are often generated through intron-to-intron ligation. To...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.