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Article

Using substrate analogues to probe the kinetic mechanism and active site of Escherichia coli MenD.

Biochemistry - 11 Oct 2011

Fang Maohai, Macova Andrea, Hanson Kimberly L, Kos Jillian, Palmer David R J

Abstract excerpt

MenD catalyzes the thiamin diphosphate-dependent decarboxylative carboligation of α-ketoglutarate and isochorismate. The enzyme is essential for menaquinone biosynthesis in many bacteria and has been proposed to be an antibiotic target. The kinetic mechanism of this enzyme has not previously been demonstrated because of the limitations of the UV-based kinetic assay. We have reported the synthesis of an...

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PMID: 21928762
DOI: 10.1021/bi201202n

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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