Article
Mutation discovery in mice by whole exome sequencing.
Genome biology - 14 Sept 2011
Fairfield Heather, Gilbert Griffith J, Barter Mary, Corrigan Rebecca R, Curtain Michelle, Ding Yueming, D'Ascenzo Mark, Gerhardt Daniel J, He Chao, Huang Wenhui, Richmond Todd, Rowe Lucy, Probst Frank J, Bergstrom David E, Murray Stephen A, Bult Carol, Richardson Joel, Kile Benjamin T, Gut Ivo, Hager Jorg, Sigurdsson Snaevar, Mauceli Evan, Di Palma Federica, Lindblad-Toh Kerstin, Cunningham Michael L, Cox Timothy C, Justice Monica J, Spector Mona S, Lowe Scott W, Albert Thomas, Donahue Leah Rae, Jeddeloh Jeffrey, Shendure Jay, Reinholdt Laura G
Abstract excerpt
We report the development and optimization of reagents for in-solution, hybridization-based capture of the mouse exome. By validating this approach in a multiple inbred strains and in novel mutant strains, we show that whole exome sequencing is a robust approach for discovery of putative mutations, irrespective of strain background. We found strong candidate mutations for the majority of mutant exomes sequenced,...
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