Article
A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1.
Genesis (New York, N.Y. : 2000) - 1 Apr 2012
Potter Leah A, Choi Eunyoung, Hipkens Susan B, Wright Christopher V E, Magnuson Mark A
Abstract excerpt
Fluorescent protein (FP) reporter alleles are useful both for identifying and purifying specific cell populations in the mouse. Here, we report the generation of mouse embryonic stem cells that contain a pancreatic and duodenal homeobox 1 (Pdx1) loxed cassette acceptor (Pdx1(LCA)) allele and the use of recombinase-mediated cassette exchange to derive mice that contain a Pdx1(CFP) (Cerulean) reporter allele. Mice...
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