Article
The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants.
The Journal of biological chemistry - 15 Jun 1990
Hamm-Alvarez S F, Sancar A, Rajagopalan K V
Abstract excerpt
Escherichia coli DNA photolyase was overproduced and purified from each of two mutant E. coli strains lacking dihydrofolate reductase. The extent of over-production in the mutants was comparable to that seen in the wild type strain. Examination of the isolated photolyase from these strains revealed that the folate cofactor, 5,10-methenyltetrahydrofolate, was present in these proteins at a level of 60-80% compared...
Topics
- Chromatography, High Pressure Liquid
- Deoxyribodipyrimidine Photo-Lyase
- Escherichia coli
- Folic Acid
- Genotype
- Mutation
- Tetrahydrofolate Dehydrogenase