Article
Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system.
Journal of bioscience and bioengineering - 1 Dec 2011
Takahashi Toru, Mizutani Osamu, Shiraishi Yohei, Yamada Osamu
Abstract excerpt
The industrial fungus Aspergillus luchuensis is used to produce a distilled spirit in Okinawa Island, Japan. Recently, the genome sequence of A. luchuensis RIB2604 (Aspergillus awamori NBRC 4314) was revealed and many functional genes are now expected to be analyzed. Gene targeting is necessary for analyzing the function of a gene; however, gene targeting frequencies in A. luchuensis are very low. To develop a...
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