Article
Next-generation sequencing of dried blood spot specimens: a novel approach to HIV drug-resistance surveillance.
Antiviral therapy - 1 Jan 2011
Ji Hezhao, Li Yang, Graham Morag, Liang Ben Binhua, Pilon Richard, Tyson Shari, Peters Geoff, Tyler Shaun, Merks Harriet, Bertagnolio Silvia, Soto-Ramirez Luis, Sandstrom Paul, Brooks James
Abstract excerpt
BACKGROUND: HIV drug-resistance (DR) surveillance in resource-limited settings can be performed using dried blood spots (DBS) because of ease of collection, transportation and storage. Analysis of pooled specimens on next-generation sequencing (NGS)-based platforms, such as the 454 pyrosequencing, is an efficient sequencing method for determining HIV DR rates. In this study, we conducted HIV DR surveillance on...
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