Article
Runx2 contributes to murine Col10a1 gene regulation through direct interaction with its cis-enhancer.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Dec 2011
Li Feifei, Lu Yaojuan, Ding Ming, Napierala Dobrawa, Abbassi Sam, Chen Yuqing, Duan Xiangyun, Wang Siying, Lee Brendan, Zheng Qiping
Abstract excerpt
We have recently shown that a 150-bp Col10a1 distal promoter (-4296 to -4147 bp) is sufficient to direct hypertrophic chondrocyte-specific reporter (LacZ) expression in vivo. More recently, through detailed sequence analysis we identified two putative tandem-repeat Runx2 binding sites within the 3'-end of this 150-bp region (TGTGGG-TGTGGC, -4187 to -4176 bp). Candidate electrophoretic mobility shift assay (EMSA),...
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