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Article

Sensitive genetic screen for protease activity based on a cyclic AMP signaling cascade in Escherichia coli.

Journal of bacteriology - 1 Dec 2000

Dautin N, Karimova G, Ullmann A, Ladant D

Abstract excerpt

We describe a genetic system that allows in vivo screening or selection of site-specific proteases and of their cognate-specific inhibitors in Escherichia coli. This genetic test is based on the specific proteolysis of a signaling enzyme, the adenylate cyclase (AC) of Bordetella pertussis. As a model system we used the human immunodeficiency virus (HIV) protease. When an HIV protease processing site, p5, was...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 11092869
DOI: 10.1128/JB.182.24.7060-7066.2000
PMCID: PMC94834

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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