Article
Stable folding core in the folding transition state of an alpha-helical integral membrane protein.
Proceedings of the National Academy of Sciences of the United States of America - 23 Aug 2011
Curnow Paul, Di Bartolo Natalie D, Moreton Kathleen M, Ajoje Oluseye O, Saggese Nicholas P, Booth Paula J
Abstract excerpt
Defining the structural features of a transition state is important in understanding a folding reaction. Here, we use Φ-value and double mutant analyses to probe the folding transition state of the membrane protein bacteriorhodopsin. We focus on the final C-terminal helix, helix G, of this seven transmembrane helical protein. Φ-values could be derived for 12 amino acid residues in helix G, most of which have low...
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