Article
Clustered-charge to alanine scanning mutagenesis of the Mal63 MAL-activator C-terminal regulatory domain.
Current genetics - 1 Dec 2003
Danzi Sara E, Bali Mehtap, Michels Corinne A
Abstract excerpt
The MAL-activator genes of Saccharomyces cerevisiae encode regulatory proteins required for the expression of the structural genes encoding maltose permease and maltase. Residues within the C-terminal region of the Mal63 protein required for negative regulation were previously identified. Evidence suggested that the C-terminal domain is also involved in positive regulatory functions, such as inducer...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Western
- Carbon Radioisotopes
- DNA Primers
- Enzyme Induction
- Maltose
- Membrane Transport Proteins
- Models, Biological
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Mutagenesis
- Mutation
- Plasmids