Article
Isochromosome 12p in non-seminoma cell lines: karyologic amplification of c-ki-ras2 without point-mutational activation.
Oncogene - 1 Apr 1990
Dmitrovsky E, Murty V V, Moy D, Miller W H, Nanus D, Albino A P, Samaniego F, Bosl G, Chaganti R S
Abstract excerpt
We examined eight human germ cell cancer lines (GCCLs) for cytogenetic abnormalities and found an isochromosome 12p, i(12p), marker in all seven male nonseminoma GCCLs, but not in the single female teratocarcinoma cell line. Southern blot analysis of these cell lines showed increased copy number...
Topics
- Blotting, Southern
- Chromosome Aberrations
- Chromosome Banding
- Chromosome Disorders
- Chromosomes, Human, Pair 12
- DNA
- DNA Probes
- DNA, Neoplasm
- Female
- Gene Amplification
- Gene Expression Regulation, Neoplastic
- Genes, ras
- Humans
- Karyotyping
- Mutation
- Oligonucleotide Probes
- Peptide Mapping
- Placenta