Article
Synthetic lethality with conditional dbp6 alleles identifies rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits.
Molecular and cellular biology - 1 Dec 1999
Kressler D, Doère M, Rojo M, Linder P
Abstract excerpt
Dbp6p is an essential putative ATP-dependent RNA helicase that is required for 60S-ribosomal-subunit assembly in the yeast Saccharomyces cerevisiae (D. Kressler, J. de la Cruz, M. Rojo, and P. Linder, Mol. Cell. Biol. 18:1855-1865, 1998). To identify factors that are functionally interacting with Dbp6p, we have performed a synthetic lethal screen with conditional dbp6 mutants. Here, we describe the cloning and...
Topics
- Alleles
- Amino Acid Sequence
- Cell Nucleolus
- DEAD-box RNA Helicases
- Fungal Proteins
- Genes, Fungal
- Molecular Sequence Data
- Mutagenesis
- Polyribosomes
- Proto-Oncogene Proteins
- RNA Helicases
- RNA Nucleotidyltransferases
- RNA, Fungal
- RNA, Ribosomal