Article
Significant contribution of arginine-112 and its positive charge of Pseudomonas putida cytochrome P-450cam in the electron transport from putidaredoxin.
Biochimica et biophysica acta - 20 Jul 1994
Nakamura K, Horiuchi T, Yasukochi T, Sekimizu K, Hara T, Sagara Y
Abstract excerpt
Cytochrome P-450cam of Pseudomonas putida is a prototype of various eukaryotic cytochrome P-450 molecules. Arg-112 located on the surface of this protein is highly conserved among various other cytochromes P-450. In this study, we constructed mutant genes for P-450cam in which Arg-112 was replaced by Gln or Glu, expressed them in Escherichia coli and purified the mutant proteins. Their enzymic activities were...
Topics
- Arginine
- Base Sequence
- Camphor
- Cytochrome P-450 Enzyme System
- Electron Transport
- Enzyme Stability
- Escherichia coli
- Ferredoxins
- Heme
- Molecular Sequence Data
- Mutation