Article
DETECTION OF DELETION α(+)-THALASSEMIA MUTATION [-α (3.7), -α (4.2)] BY QUANTITATIVE PCR ASSAY.
The Southeast Asian journal of tropical medicine and public health - 1 Jan 2015
Seeratanachot Teerapat, Shimbhu Dawan, Charoenkwan Pimlak, Sanguansermsri Torpong
Abstract excerpt
In Thailand, Hb H (α0(-thal/α(+)-thal) disease is highly prevalent. We designed 3 primer sets (A, B and C) to detect -α (3.7) and -α (4.2) deletion types of α(+)-thal by quantitative (q)PCR. The A and C primer sets were used to amplify DNA sequences at the 3' terminal regions of HBA2 and HBA1 gene, respectively, and the B primer set was used to amplify an upstream DNA sequence at the 5' flanking region of HBA1...
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