Article
Nuclear mutation restores the reduced CO2/O2 specificity of ribulosebisphosphate carboxylase/oxygenase in a temperature-conditional chloroplast mutant of Chlamydomonas reinhardtii.
Archives of biochemistry and biophysics - 15 Nov 1990
Chen Z X, Green D, Westhoff C, Spreitzer R J
Abstract excerpt
The Chlamydomonas reinhardtii temperature-sensitive mutant 68-4PP results from a mutation within the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. When grown at the permissive temperature (25 degrees C), the mutant has a reduced level of holoenzyme protein, and the purified enzyme has a lower CO2/O2 specificity than the wild-type enzyme. At the nonpermissive...
Topics
- Base Sequence
- Carbon Dioxide
- Cell Nucleus
- Chlamydomonas
- Chloroplasts
- Cloning, Molecular
- Codon
- Crosses, Genetic
- Kinetics
- Molecular Sequence Data
- Mutation