Article
Heterologous expression of human 5-aminolevulinate dehydratase in Saccharomyces cerevisiae.
Current genetics - 1 Jan 1990
Schauer W E, Mattoon J R
Abstract excerpt
A cDNA coding for human 5-amino-levulinate dehydratase was placed in a yeast expression vector under the control of the GAL10 promoter. The resulting multicopy plasmid was then used to transform a yeast mutant which contains a defective hem2 gene coding for 5-aminolevulinate dehydratase. Expressi...
Topics
- Alleles
- Cytochromes
- DNA
- Gene Expression Regulation, Enzymologic
- Genes
- Humans
- Mutation
- Plasmids
- Porphobilinogen Synthase
- Promoter Regions, Genetic
- Saccharomyces cerevisiae
- Transformation, Genetic