Article
Heterologous expression of human 5-aminolevulinate dehydratase in Saccharomyces cerevisiae.
Current genetics - 1 Jan 1990
Schauer W E, Mattoon J R
Abstract excerpt
A cDNA coding for human 5-amino-levulinate dehydratase was placed in a yeast expression vector under the control of the GAL10 promoter. The resulting multicopy plasmid was then used to transform a yeast mutant which contains a defective hem2 gene coding for 5-aminolevulinate dehydratase. Expression of the human cDNA was shown in four ways: (1) restoration of normal growth on glycerol/galactose as primary carbon...
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