Characterization of cDNAs coding for glucose phosphate isomerase and phosphoglycerate kinase in Chinese hamster ovary cell line CHO-K1 and identification of defects in R1.1.7, a glycolysis-deficient variant of CHO-K1.

Abstract excerpt

Full-length cDNAs for glucose phosphate isomerase (GPI) and phosphoglycerate kinase (PGK) of the Chinese hamster ovary cell line CHO-K1 have been characterized using RT-PCR and cycle sequencing of the PCR-amplified templates. Mutations in both genes have been identified in a glycolysis-deficient Chinese hamster ovary cell line, R1.1.7, derived from CHO-K1 cells.

Topics

• Animals
• Base Sequence
• CHO Cells
• Cricetinae
• DNA, Complementary
• Female
• Glucose-6-Phosphate Isomerase
• Molecular Sequence Data
• Mutation
• Phosphoglycerate Kinase
• Polymerase Chain Reaction