Article
Detection of the JAK2 mutation in myeloproliferative neoplasms by asymmetric PCR with unlabeled probe and high-resolution melt analysis.
Journal of clinical laboratory analysis - 1 Jan 2011
Cao Hong-Cui, Lin Jiang, Qian Jun, Yao Dong-Ming, Li Yun, Yang Jing, Chen Qin, Chai Hai-Yan, Xiao Gao-Fei
Abstract excerpt
BACKGROUND: Several methods have been established to detect the JAK2 V617F mutation, a frequent event involved in the pathogenesis of myeloproliferative neoplasms (MPNs). High-resolution melt (HRM) analysis is a newly established technique without the requirement of any gel-based post-PCR handling. METHODS: An asymmetric PCR with unlabeled specific probe was developed and combined to HRM analysis o screen for...
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