Article
Transfer and expression of the lacZ gene in rat brain neurons mediated by herpes simplex virus mutants.
The New biologist - 1 Aug 1990
Chiocca E A, Choi B B, Cai W Z, DeLuca N A, Schaffer P A, DiFiglia M, Breakefield X O, Martuza R L
Abstract excerpt
Three mutants of herpes simplex virus type 1 (HSV-1) were used to deliver and express the Escherichia coli lacZ gene in cells of the rat central nervous system. Because the lacZ gene was inserted in place of the genes encoding one of the immediate-early viral proteins ICP0 or ICP4 or the early viral protein thymidine kinase, these mutants were compromised or defective in their ability to replicate. All mutant...
Topics
- Animals
- Brain
- Cell Line
- Escherichia coli
- Gene Expression
- Hybridomas
- Immediate-Early Proteins
- Lac Operon
- Mutation
- Neurons
- Promoter Regions, Genetic