Article
Comparative analysis of CE-SSCP to standard RFLP-CE-FLA method in quantification of known viral variants within an RNA virus quasispecies.
Electrophoresis - 1 Jul 2011
Gulija Tanja Košutić, Ivancic-Jelecki Jelena, Santak Maja, Forcic Dubravko
Abstract excerpt
RNA viruses display the highest replication error rate in our biosphere, leading to highly diverse viral populations termed quasispecies. The gold standard method for detection and quantification of variants in a quasispecies is cloning and sequencing, but it is expensive, laborious and time consuming. Therefore, other mutation detection approaches, including SSCP, are often used. In this study, we demonstrate...
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