Article
Molecular identification of mumps virus genotypes from clinical samples: standardized method of analysis.
Journal of clinical microbiology - 1 Apr 2005
Palacios G, Jabado O, Cisterna D, de Ory F, Renwick N, Echevarria J E, Castellanos A, Mosquera M, Freire M C, Campos R H, Lipkin W I
Abstract excerpt
A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospinal fluid (CSF)...
Topics
- Amino Acid Sequence
- Base Sequence
- Child
- Child, Preschool
- Encephalitis, Viral
- Female
- Genotype
- Humans
- Infant
- Infant, Newborn
- Male
- Meningitis, Aseptic
- Molecular Sequence Data
- Mumps