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Article

Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products.

Applied and environmental microbiology - 1 Jan 2003

Courtois Sophie, Cappellano Carmela M, Ball Maria, Francou Francois-Xavier, Normand Philippe, Helynck Gérard, Martinez Asuncion, Kolvek Steven J, Hopke Joern, Osburne Marcia S, August Paul R, Nalin Renaud, Guérineau Michel, Jeannin Pascale, Simonet Pascal, Pernodet Jean-Luc

Abstract excerpt

To further explore possible avenues for accessing microbial biodiversity for drug discovery from natural products, we constructed and screened a 5,000-clone "shotgun" environmental DNA library by using an Escherichia coli-Streptomyces lividans shuttle cosmid vector and DNA inserts from microbes derived directly (without cultivation) from soil. The library was analyzed by several means to assess diversity, genetic...

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Identifiers

PMID: 12513976
DOI: 10.1128/AEM.69.1.49-55.2003
PMCID: PMC152451

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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