Article
Partial deletion of the Rhizobium phaseoli CFN23 symbiotic plasmid implies a concomitant amplification of plasmid DNA sequences.
Molecular microbiology - 1 Jan 1991
Soberón-Chávez G, Nájera R, Calva E, Fernández M
Abstract excerpt
Rhizobium leguminosarum biovar phaseoli CFN23 loses its ability to nodulate beans at a high frequency because of a deletion of part of its symbiotic (pSym) plasmid (Soberón-Chávez et al., 1986). We report here that at least 80 kb of pSym are deleted upon loss of the symbiotic phenotype; the deletion removes the nitrogenase structural nifHDK and the common nodABC genes. The size of the deleted pSym is not reduced,...
Topics
- Blotting, Southern
- Chromosome Deletion
- DNA, Bacterial
- Fabaceae
- Gene Amplification
- Genes, Bacterial
- Nitrogen Fixation
- Nitrogenase
- Phenotype
- Plants, Medicinal
- Plasmids