Article
Chromosome mapping in Pseudomonas syringae pv. syringae strain PS224.
Journal of general microbiology - 1 Jul 1990
Nordeen R O, Holloway B W
Abstract excerpt
A conjugation system for mapping the chromosome of Pseudomonas syringae pv. syringae PS224 has been developed using the IncP-10 plasmid R91-5; pMO22, a Tn501-loaded derivative of R91-5; and pMO75, R91-5 loaded with Tn5. Nine different donor origins were identified with R91-5 and pMO22. By insertion of Tn5 into various sites of the chromosome, an additional six donor origins were available using pMO75 as the donor...
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