Article
Determining the extremes of the cellular NAD(H) level by using an Escherichia coli NAD(+)-auxotrophic mutant.
Applied and environmental microbiology - 1 Sept 2011
Zhou Yongjin, Wang Lei, Yang Fan, Lin Xinping, Zhang Sufang, Zhao Zongbao K
Abstract excerpt
NAD (NAD(+)) and its reduced form (NADH) are omnipresent cofactors in biological systems. However, it is difficult to determine the extremes of the cellular NAD(H) level in live cells because the NAD(+) level is tightly controlled by a biosynthesis regulation mechanism. Here, we developed a strategy to determine the extreme NAD(H) levels in Escherichia coli cells that were genetically engineered to be NAD(+)...
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