Article
[Effects of overexpression of atpA, purHD and ndh on the growth of Escherichia coli DH5alpha].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Aug 2008
Zhang Yanjun, Zhang Xiaoyun, Li Zhimin, Ye Qin
Abstract excerpt
OBJECTIVE: To study the mechanism of the differences in cell growth and acetate production between Escherichia coli DH5alpha and its acetate-tolerant mutant DA19. METHODS: Based on our previous analysis of the proteome and mass and energy balances for both strains, we first amplified the genes of atpIBEFHAGDC, purHD, and ndh, their regulation regions, as well as the regulation region of nuoA-N in both strains by...
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