Article
Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp. PCC 6803.
Journal of plant physiology - 20 Oct 2016
Ishikawa Yuuma, Miyagi Atsuko, Haishima Yuto, Ishikawa Toshiki, Nagano Minoru, Yamaguchi Masatoshi, Hihara Yukako, Kawai-Yamada Maki
Abstract excerpt
NAD kinase (NADK) phosphorylates NAD(H) to NADP(H). The enzyme has a crucial role in the regulation of the NADP(H)/NAD(H) ratio in various organisms. The unicellular cyanobacterium Synechocystis sp. PCC 6803 possesses two NADK-encoding genes, sll1415 and slr0400. To elucidate the metabolic change in NADK-deficient mutants growing under photoautotrophic conditions, we conducted metabolomic analysis using capillary...
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