Action of a transposable element in coding sequence fusions.

doi:10.1093/genetics/126.2.293

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Article

Action of a transposable element in coding sequence fusions.

Genetics - 1 Oct 1990

Shapiro J A, Leach D

Abstract excerpt

The original Casadaban technique for isolating fused cistrons encoding hybrid beta-galactosidase proteins used a Mucts62 prophage to align the upstream coding sequence and lacZ prior to selection. Kinetic analysis of araB-lacZ fusion colony emergence indicated that the required DNA rearrangements...

Topics

Base Sequence
Cloning, Molecular
DNA Transposable Elements
Exons
Genes, Bacterial
Genetic Complementation Test
Kinetics
Models, Genetic
Molecular Sequence Data
Mutation

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