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Article

Action of a transposable element in coding sequence fusions.

Genetics - 1 Oct 1990

Shapiro J A, Leach D

Abstract excerpt

The original Casadaban technique for isolating fused cistrons encoding hybrid beta-galactosidase proteins used a Mucts62 prophage to align the upstream coding sequence and lacZ prior to selection. Kinetic analysis of araB-lacZ fusion colony emergence indicated that the required DNA rearrangements were regulated and responsive to conditions on selection plates. This has been cited as an example of "directed...

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Identifiers

PMID: 2174011
DOI: 10.1093/genetics/126.2.293
PMCID: PMC1204183

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