Blockage to exonuclease III digestion in the chromatin of Saccharomyces cerevisiae maps to the in vitro--determined binding site of a trans-acting regulatory factor.

Abstract excerpt

A series of transposable element-induced mutations at the HIS4 locus in Saccharomyces cerevisiae have been attributed to the transposition of a Ty element into the 5' regulatory region of this gene. Various Ty-containing His+ revertants have been isolated and the HIS4/Ty junction region sequenced. The only difference found in this region between a His- and a weak His+ strain was a single point mutation, an A----G...

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