Article
Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.
Journal of bacteriology - 1 May 1990
Komano T, Funayama N, Kim S R, Nisioka T
Abstract excerpt
To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques followed by double homologous recombination in vivo. A plasmid designated pKK609 and bearing a 56.7-kilobase R64 sequence was the smallest transferable plasmid. A plasmid designated pKK610 and no longer possessing the...
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