Article
Use of an intergenic region in Pseudomonas syringae pv. syringae B728a for site-directed genomic marking of bacterial strains for field experiments.
Applied and environmental microbiology - 1 Aug 2001
Hirano S S, Willis D K, Clayton M K, Upper C D
Abstract excerpt
To construct differentially-marked derivatives of our model wild-type strain, Pseudomonas syringae pv. syringae B728a (a causal agent of bacterial brown spot disease in snap bean plants), for field experiments, we selected a site in the gacS-cysM intergenic region for site-directed insertion of antibiotic resistance marker cassettes. In each of three field experiments, population sizes of the site-directed...
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