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Article

One amino acid change produces a high affinity cGMP-binding site in cAMP-dependent protein kinase.

The Journal of biological chemistry - 25 Sept 1990

Shabb J B, Ng L, Corbin J D

Abstract excerpt

Discrimination between cAMP and cGMP is a critical feature of cAMP- and cGMP-dependent protein kinases. An alanine/threonine difference in the cyclic nucleotide-binding sites has been proposed to provide a structural basis for this functional distinction. Site-directed mutagenesis of this alanine to a threonine in a cAMP-binding site of cAMP kinase produced a mutant with markedly increased cGMP affinity as...

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PMID: 2168874

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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