Article
One amino acid change produces a high affinity cGMP-binding site in cAMP-dependent protein kinase.
The Journal of biological chemistry - 25 Sept 1990
Shabb J B, Ng L, Corbin J D
Abstract excerpt
Discrimination between cAMP and cGMP is a critical feature of cAMP- and cGMP-dependent protein kinases. An alanine/threonine difference in the cyclic nucleotide-binding sites has been proposed to provide a structural basis for this functional distinction. Site-directed mutagenesis of this alanine...
Topics
- Alanine
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cattle
- Cyclic AMP
- Cyclic GMP
- Kinetics
- Macromolecular Substances
- Male
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Protein Kinases
- Testis
- Threonine