Article
Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry.
Biochemistry - 17 Jul 1990
Westrick J A, Peters K S, Ropp J D, Sligar S G
Abstract excerpt
The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns. The enthalpy of the intermediate increases by approximately 7 kcal/mol upon replacing...
Topics
- Acoustics
- Animals
- Arginine
- Base Sequence
- Calorimetry
- Carbon Monoxide
- Chemical Phenomena
- Chemistry, Physical
- Diffusion
- Molecular Sequence Data
- Mutation
- Myoglobin