The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

Article

The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

The Journal of biological chemistry - 15 Jul 1990

Egeberg K D, Springer B A, Sligar S G, Carver T E, Rohlfs R J, Olson J S

Abstract excerpt

Site-directed mutants of sperm whale myoglobin were prepared to probe the functional role of the highly conserved distal pocket valine residue, Val68(E11). This amino acid was replaced with Ala, Ile, and Phe to examine the effects of the side chain volume at position 68 on ligand binding. Three d...

Topics

Amino Acid Sequence
Animals
Carbon Dioxide
Genes, Synthetic
Kinetics
Ligands
Mutation
Myoglobin
Protein Binding
Protein Conformation
Valine
Whales

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