Article
Contributions of residue 45(CD3) and heme-6-propionate to the biomolecular and geminate recombination reactions of myoglobin.
Biochemistry - 14 May 1991
Carver T E, Olson J S, Smerdon S J, Krzywda S, Wilkinson A J, Gibson Q H, Blackmore R S, Ropp J D, Sligar S G
Abstract excerpt
Overall association and dissociation rate constants were measured at 20 degrees C for O2, CO, and alkyl isocyanide binding to position 45 (CD3) mutants of pig and sperm whale myoglobins and to sperm whale myoglobin reconstituted with protoheme IX dimethyl ester. In pig myoglobin, Lys45(CD3) was replaced with Arg, His, Ser, and Glu; in sperm whale myoglobin, Arg45(CD3) was replaced with Ser and Gly. Intramolecular...
Topics
- Animals
- Binding, Competitive
- Carbon Monoxide
- Heme
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Myoglobin
- Nitrogen Oxides
- Oxygen
- Protein Conformation