Interruption of capsule production in Streptococcus pneumonia serotype 3 by insertion of transposon Tn916.

doi:10.1128/iai.58.9.3135-3138.1990

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Article

Interruption of capsule production in Streptococcus pneumonia serotype 3 by insertion of transposon Tn916.

Infection and immunity - 1 Sept 1990

Watson D A, Musher D M

Abstract excerpt

Transposon Tn916 mutagenesis was used to produce mutant strains of Streptococcus pneumoniae serotype 3 that lacked only a polysaccharide capsule. Southern blotting, DNA-DNA hybridization, and immunochemical analyses demonstrated that the presence of a single copy of Tn916 was sufficient to produc...

Topics

Animals
Cloning, Molecular
Colony Count, Microbial
DNA Transposable Elements
Female
Genes, Bacterial
Mice
Mice, Inbred Strains
Mutation
Pneumococcal Infections
Polysaccharides, Bacterial
Streptococcus pneumoniae
Virulence

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