Article
Vibrational spectroscopy of bacteriorhodopsin mutants: evidence for the interaction of proline-186 with the retinylidene chromophore.
Biochemistry - 26 Jun 1990
Rothschild K J, He Y W, Mogi T, Marti T, Stern L J, Khorana H G
Abstract excerpt
Fourier-transform infrared difference spectroscopy has been used to study the role of the three membrane-embedded proline residues, Pro-50, Pro-91, and Pro-186, in the structure and function of bacteriorhodopsin. All three prolines were replaced by alanine and glycine; in addition, Pro-186 was ch...
Topics
- Amino Acid Sequence
- Asparagine
- Bacteriorhodopsins
- Fourier Analysis
- Molecular Sequence Data
- Mutation
- Photochemistry
- Proline
- Protein Conformation
- Protons
- Retinal Pigments
- Schiff Bases
- Spectrophotometry, Infrared
- Spectrum Analysis
- Tyrosine
- Valine
- Vibration