Article
Vibrational spectroscopy of bacteriorhodopsin mutants. Evidence for the interaction of aspartic acid 212 with tyrosine 185 and possible role in the proton pump mechanism.
The Journal of biological chemistry - 5 Oct 1990
Rothschild K J, Braiman M S, He Y W, Marti T, Khorana H G
Abstract excerpt
The role of Asp-212 in the proton pumping mechanism of bacteriorhodopsin (bR) has been studied by a combination of site-directed mutagenesis and Fourier transform infrared difference spectroscopy. Difference spectra were recorded at low temperature for the bR----K and bR----M photoreactions of th...
Topics
- Amino Acid Sequence
- Aspartic Acid
- Bacteriorhodopsins
- Fourier Analysis
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Schiff Bases
- Spectrophotometry, Infrared
- Vibration