FTIR difference spectroscopy of the bacteriorhodopsin mutant Tyr-185-->Phe: detection of a stable O-like species and characterization of its photocycle at low temperature.

doi:10.1021/bi00060a021

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Article

FTIR difference spectroscopy of the bacteriorhodopsin mutant Tyr-185-->Phe: detection of a stable O-like species and characterization of its photocycle at low temperature.

Biochemistry - 9 Mar 1993

He Y, Krebs M P, Fischer W B, Khorana H G, Rothschild K J

Abstract excerpt

Fourier transform infrared difference spectroscopy has been used to study the photocycle of the mutant Tyr-185-->Phe expressed in native Halobacterium halobium and isolated as intact purple membrane fragments. We find several changes in the low-temperature bR-->K, bR-->L, and bR-->M FTIR differen...

Topics

Bacteriorhodopsins
Cold Temperature
Fourier Analysis
Halobacterium salinarum
Mutation
Phenylalanine
Photochemistry
Spectrophotometry, Infrared
Tyrosine

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