Article
FTIR difference spectroscopy of the bacteriorhodopsin mutant Tyr-185-->Phe: detection of a stable O-like species and characterization of its photocycle at low temperature.
Biochemistry - 9 Mar 1993
He Y, Krebs M P, Fischer W B, Khorana H G, Rothschild K J
Abstract excerpt
Fourier transform infrared difference spectroscopy has been used to study the photocycle of the mutant Tyr-185-->Phe expressed in native Halobacterium halobium and isolated as intact purple membrane fragments. We find several changes in the low-temperature bR-->K, bR-->L, and bR-->M FTIR difference spectra of Y185F relative to wild-type bR which are not directly related to the absorption bands associated with...
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