[Identification of a novel allele HLA-B*15:129 by polymerase chain reaction with allele group-specific primers].

doi:10.3760/cma.j.issn.1003-9406.2011.03.012

Article

[Identification of a novel allele HLA-B*15:129 by polymerase chain reaction with allele group-specific primers].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Jun 2011

Han Zhe-dong, Wang Wei, Zhang Wei, He Jun-jun, Chen Nan-ying, Zhu Fa-ming, Lv Hang-jun, Yan Li-xing

Abstract excerpt

OBJECTIVE: To analyze the sequence of the exons 2-4 of human leukocyte antigen (HLA) novel allele HLA-B*15:129. METHODS: DNA of the proband was extracted from whole blood by commercial DNA extraction kit. The amplification for HLA-B exons 2-4 was performed separately by polymerase chain reaction (PCR) with allele group specific primers. The PCR products were digested with enzymes and then directly sequenced for...

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[Identification of a novel allele HLA-B*15:129 by polymerase chain reaction with allele group-specific primers].

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