Article
[Nucleotide sequence analysis of A novel HLA-B*15:124 allele confirmed].
Zhongguo shi yan xue ye xue za zhi - 1 Dec 2010
Wang Wei, Zhang Wei, Han Zhe-Dong, He Jun-Jun, Chen Nan-Ying, Zhu Fa-Ming, Lü Hang-Jun, Yan Li-Xing
Abstract excerpt
This study was purposed to investigate the nucleotide sequences of a novel HLA-B*15:124 allele and its molecular mechanism. The genomic DNA from whole blood was extracted by using commercial DNA extraction kit. The sequences of exon 2, 3 and 4 of HLA-B locus in the proband were amplified by PCR with group-specific primers, the PCR products were purified by enzymes digestion, then exon 2 to 4 of HLA-B locus for...
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