[Identification and sequence analysis of a novel human leukocyte antigen allele B*54:09].

doi:10.3760/cma.j.issn.1003-9406.2012.01.022

Article

[Identification and sequence analysis of a novel human leukocyte antigen allele B*54:09].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Feb 2012

Zhang Xu, Jiang Shu-yi, Li Xiao-feng, Zhang Kun-lian, Chen Yang, Liu Xian-zhi, Li Jian-ping

Abstract excerpt

OBJECTIVE: To identify a novel human leukocyte antigen(HLA) allele in Chinese population. METHODS: HLA typing was carried out with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP). The HLA-B exons 1-7 of the proband were amplified and the product was cloned using a TOPO TA cloning sequencing kit to separate the two alleles. Both strands of exons 2 and 3 of selected colonies were...

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[Identification and sequence analysis of a novel human leukocyte antigen allele B*54:09].

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