Article
[Sequence analysis of a novel HLA-A*3308 allele].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Jun 2006
Lv Qin-feng, Zhang Wei, He Ji, Wang Wei, Han Zhe-dong, Zhu Fa-ming, Yan Li-xing
Abstract excerpt
OBJECTIVE: To investigate the molecular genetics basis for HLA novel allele HLA-A*3308 in Chinese population. METHODS: DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 1-8 of the proband was preformed by PCR and the amplified product was cloned with TOPO cloning sequencing kit to split the two alleles apart. Both strands of exons 2, 3 and 4 of chosen clones were...
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