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Article

A synthetic HIV-1 subtype C backbone generates comparable PR and RT resistance profiles to a subtype B backbone in a recombinant virus assay.

PloS one - 1 Jan 2011

Nauwelaers David, Van Houtte Margriet, Winters Bart, Steegen Kim, Van Baelen Kurt, Chi Ellen, Zhou Mimi, Steiner Derek, Bonesteel Rachelle, Aston Colin, Stuyver Lieven J

Abstract excerpt

In order to determine phenotypic protease and reverse transcriptase inhibitor-associated resistance in HIV subtype C virus, we have synthetically constructed an HIV-1 subtype C (HIV-1-C) viral backbone for use in a recombinant virus assay. The in silico designed viral genome was divided into 4 fragments, which were chemically synthesized and joined together by conventional subcloning. Subsequently,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 21629677
DOI: 10.1371/journal.pone.0019643
PMCID: PMC3101197

Genes

CPE

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