Robust and efficient genotyping of factor V and prothrombin alleles using amplicon high-resolution melting and synthetic DNA controls: validation of the test with three different commercial chemistries.

Abstract excerpt

OBJECTIVES: Developing robust HRM (amplicon High Resolution Melting) analysis valid for different commercial reaction mixes, using synthetic control DNA samples and the RotorGeneQ (Qiagen) instrument. DESIGN AND METHODS: 126 samples were analyzed for the presence of the factor Leiden and the 20210G>A prothrombin alleles. The four alleles were cloned and used to prepare synthetic controls. RESULTS: All mutant...

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