A general method for the transfer of plasmid-borne mutant alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda: transfer of pqq genes.

Abstract excerpt

A generally applicable method is described for reintroduction of mutant plasmid-borne alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda. We used this method to make stable chromosomal transposon insertions in genes for biosynthesis of pyrroloquinoline quinone in K. pneumoniae.

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alleles
• Share to Bacteriophage lambda
• Share to Chromosomes, Bacterial