Identification of RNA pseudoknot-binding ligand that inhibits the -1 ribosomal frameshifting of SARS-coronavirus by structure-based virtual screening.

Abstract excerpt

Programmed -1 ribosomal frameshifting (-1 RF) is an essential regulating mechanism of translation used by SARS-CoV (severe acute respiratory syndrome coronavirus) to synthesize the key replicative proteins encoded by two overlapping open reading frames. The integrity of RNA pseudoknot stability and structure in the -1 RF site is important for efficient -1 RF. Thus, small molecules interacting with high affinity...

Topics

• Antiviral Agents
• Base Sequence
• Drug Evaluation, Preclinical
• Frameshifting, Ribosomal
• Ligands
• Models, Molecular
• Mutation
• Nucleic Acid Conformation
• RNA, Viral
• Severe acute respiratory syndrome-related coronavirus
• Transcription, Genetic
• User-Computer Interface