Article
Identification of C3b-binding regions on herpes simplex virus type 2 glycoprotein C.
Journal of virology - 1 May 1990
Seidel-Dugan C, Ponce de Leon M, Friedman H M, Eisenberg R J, Cohen G H
Abstract excerpt
Glycoprotein C from herpes simplex viruses types 1 and 2 (gC-1 and gC-2) acts as a receptor for the C3b fragment of the third component of complement. Our goal is to identify domains on gC involved in C3b receptor activity. Here, we used in-frame linker-insertion mutagenesis of the cloned gene for gC-2 to identify regions of the protein involved in C3b binding. We constructed 41 mutants of gC-2, each having a...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Base Sequence
- Cells, Cultured
- Cloning, Molecular
- Complement C3b
- Genes, Viral
- Mice
- Models, Structural
- Molecular Sequence Data