Article
Mutations of the human papillomavirus type 16 E7 gene that affect transformation, transactivation and phosphorylation by the E7 protein.
The Journal of general virology - 1 Apr 1990
Storey A, Almond N, Osborn K, Crawford L
Abstract excerpt
The human papillomavirus type 16 (HPV-16) E7 gene cooperates with an activated ras oncogene to transform primary rodent cells and is important in the immortalization of cervical keratinocytes. We have generated a series of point mutations within the E7 gene and show that mutation of residues serine 31 and serine 71 affect the phosphorylation of the E7 protein, but do not alter its ability to cooperate with ras....
Topics
- Animals
- Cell Line
- Cell Transformation, Neoplastic
- Cell Transformation, Viral
- Cysteine
- DNA Mutational Analysis
- Humans
- Mutation
- Oncogene Proteins, Viral
- Papillomaviridae
- Papillomavirus E7 Proteins